Protective effect of tanshinone ⅡA on undulatory high glucose injuried human umbilical vein endothelial cell
10.13699/j.cnki.1001-6821.2016.04.019
- VernacularTitle:丹参酮ⅡA对波动性高糖体外诱导人脐静脉内皮细胞损伤保护作用的机制研究
- Author:
Jing ZHANG
1
;
Da-Xin WANG
;
Sheng-Hu HE
;
Jun-Sheng LU
Author Information
1. 中南大学 湘雅二医院 心内科
- Keywords:
human umbilical vein endothelial cell;
undulatory high glucose;
tanshinone ⅡA;
oxidative stress;
Rho/Rho kinase system
- From:
The Chinese Journal of Clinical Pharmacology
2016;(4):346-348
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influences of tanshinone ⅡA on undulatory high glucose injuried human umbilical vein endothelial cells ( HUVEC ) activity, malondialdehyde ( MDA ) , superoxide dismutase (SOD), nitric oxide (NO), nitric oxide synthase (NOS) and Rho/Rho kinase Ⅰ( ROCKⅠ) mRNA and to explore its mechanism.Methods HUVEC was divided into six groups , normal control group was given 5.50 mmol· L-1 glucose 200 μL for 48 h.Injury group was given 5.50, 20.00 mmol· L-1 glucose 200μL for 48 h.Low, medium and high dose groups were based on the treatment of injury group , and taken keep 10.00 , 30.00 , 50.00 μg· mL-1 tanshinone ⅡA 200 μL for 48 h, re-spectively.Positive control group was based on the treatment of injury group , and given 100.00 mg· L-1 vitamin C 200μL for 48 h.The level of MDA, SOD, NO, NOS and ROCKⅠ mRNA were compared between six groups.Results The amount of SOD , NO and NOS in the normal control group , positive control group , low, medium and high dose groups were significantly higher than that of injury group, the amount of MDA and ROCKⅠ mRNA content were significantly less than that of injury group , statistically significant ( P<0.05 ) except low dose group , of which high dose group was more obvious ( P<0.05 ).Conclusion Tanshinone ⅡA has a protective effect on the HUVEC injury induced by a undulatory high sugar in vitro of volatility , which can increase the level of SOD , NO and NOS, and decrease the content of MDA and ROCKⅠ mRNA.So the mechanism may be related to remove active oxygen , reduce lipid peroxidation , enhance the vitality of endothelial cell antioxidant enzyme system , and through the Rho/Rho kinase system to refrain NOS expression , which can affect NO se-cretion and so on.
