Effects of interleukin 1βon the intracellular cholesterol contents of HepG2 cells treated by compactin
10.13699/j.cnki.1001-6821.2016.04.018
- VernacularTitle:白细胞介素1β对美伐他汀处理的 HepG2细胞胞内胆固醇含量的影响
- Author:
Wei ZHOU
1
;
Pei WANG
;
Ya-Xi CHEN
;
Xiong-Zhong RUAN
;
Lei ZHAO
Author Information
1. 重庆医科大学 脂糖代谢性疾病重庆市重点实验室
- Keywords:
compactin;
interleukin-1β;
cholesterol;
hepg 2 cell
- From:
The Chinese Journal of Clinical Pharmacology
2016;(4):343-345
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigated the effects of interleukin -1β( IL-1β) on the intracellular cholesterol contents of HepG 2 cells treated by compactin.Methods HepG2 cells were treated with different con-centrations of compactin (0, 1, 10, 50, 100 μmol· L-1 ) in the absence or presence of 20 ng· mL-1 IL-1βfor 24 hours.Commercial kits was used to evaluated the enzymatic activity of 3-hydroxy-3-methyl-glu-taryl (HMG) -CoA reductase ( HMG-CoA -R).Real -time PCR was used to determine the mRNA of low density lipoprotein ( LDL) rece-puter.Then HepG2 cells treated with 100 μmol · L-1 compactin in the absence or presence of 20 ng· mL-1 IL-1βwere chose to evaluated the content of intracellular cholesterol by commercial kits , real -time PCR was used to determine the mRNA of acyl coenzyme A -cholesterol acyl-transferase ( ACAT ).Results Compactin dose dependently reduced HMG-CoA -R enzymatic activity.IL -1βupregulated the enymatic activity of HMG-CoA reductase , LDL receptor , and the expression of acyl -coenzyme A -cholesterol acyltransferase mRNA , which was accompanied with increased intracellular cholesterol ester contents. Conclusion Inflammatory cytokine disrupted the suppressive effect of compactin on the HMG -CoA-R enzymatic ac-tivity in HepG2 cells and increased the expression of ACAT and LDL receptor and may contribute to hepatic cholesterol accumulation under inflammatory conditions.
