Protective effects of tetramethylpyrazine on isoprenaline-induced acute myocardial ischemia
10.13699/j.cnki.1001-6821.2016.04.017
- VernacularTitle:川芎嗪对异丙肾上腺素诱导的小鼠急性心肌缺血的保护作用
- Author:
Rui-Fang LI
1
;
Hong-Wei WANG
;
Shi-Chao ZHU
;
Jun-Min FU
;
Hong-Chao CHENG
;
Hong-Yun WANG
Author Information
1. 河南科技大学
- Keywords:
tetramethylpyrazine;
myocardial ischemia;
isoproterenol;
cardiac enzymes;
oxidative stress
- From:
The Chinese Journal of Clinical Pharmacology
2016;(4):340-342,353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects and mecha-nism of tetramethylpyrazine ( TMP ) on isoproterenol ( ISO ) -induced acute myocardial ischemia.Methods BALB/c mice were randomly divided into 6 groups:normal group ( saline 10 mg· kg -1 · d-1 ) , model group ( ISO 3 mg· kg -1 · d-1 , three times for three days ) , control group ( propranolol 0.4 mg · kg -1 · d-1 ) , different dose TMP group (60, 30, 15 mg· kg -1· d-1), 10 mice in each group.All groups were administered drugs for 7 days.Then ISO was given intraperitoneally to establish acute myocardial ischemia model.Tetrazolium chloride ( TTC ) staining was used to determine focal myocardial ischemia and hematoxylin-eosin ( HE ) staining was performed in histopathological examination.MB isoenzyme of creatine kinase ( CK -MB ) , lactate dehydrogenase ( LDH) levels and hydrogen peroxide ( H2 O2 ) , total an-tioxidant capacity ( T -AOC ) of serum were detected.Results TMP obviously attenuated abnormal electrocardiogram with ST -segment elevation , myocardial damage and ischemia region area in model mice.Compared with model group [ CK -MB: ( 1562.46 ± 138.92 ) U · L-1; LDH:(6973.28 ±285.50 ) U· L-1; T-AOC: ( 2.99 ±1.25 ) U · mL-1;H2 O2:( 92.52 ±11.42 ) mmol · L-1 ] , TMP group significantly decreased the levels of serum [ CK -MB:( 605.81 ±117.72 ), ( 838.67 ±159.73 ), (1264.37 ±134.17) U· L-1);LDH:(2661.79 ±229.27),(5776.16 ±331.39), (6014.59 ±291.56) U· L-1);H2O2:(31.98 ±7.38), (67.06 ±18.43), (86.62 ±13.41) mmol· L-1] and increased T-AOC [(7.54 ±2.51), (5.80 ±2.53 ) , (4.93 ±2.21 ) U · mL-1 ] levels of myocardial ischemia mice ( P <0.05 or P <0.01 ) . Conclusion TMP would alleviate Iso-induced acute myocardial ischemia , which may be associated with the decrease of cardiac enzymes and its protective effects against oxidative stress.
