Quality control of Angelica dahurica by standardized reference extract qualification and single compound quantitative evaluation
10.13699/j.cnki.1001-6821.2016.04.015
- VernacularTitle:对照提取物定性和单标定量相结合的白芷药材质量控制研究
- Author:
De-Qiang LI
1
;
Li-Guang DUAN
;
Xu-Guang ZHENG
;
Rong-Yuan DAI
;
Zhi-Qing ZHANG
Author Information
1. 河北医科大学 第二医院 药学部
- Keywords:
Angelica dahurica;
quality control;
standardized reference extract;
single compound quantitative evaluation
- From:
The Chinese Journal of Clinical Pharmacology
2016;(4):333-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective Quality control of Angelica dahurica by standard-ized reference extract qualification and single compound quantitative eval-uation.Methods The samples were analyzed by HPLC after pressurized liquid extraction ( PLE).The chromatographic conditions were as follow-ing:The column was ACE UltraCore C 18 (50 mm ×2.1 mm, 2.5 μm);the mobile phase consisted of acetonitrile -water using gradient elution;the flow rate was 1.0 mL · min-1 and the column temperature was 30℃.The detection wavelength was set at 310 nm and the sample injection volume was 5μL.The method was validated by investigating its specifici-ty, linearities and limit of detection , precision , extraction recovery , sta-bility and repeatability.Results Five components including bergapten , oxypeucedanin , imperatorin, cinidilin and isoimperatorin were deter-mined by HPLC.The linearities of them were good ( r≥0.999 4 ) in the concentration ranges of 0.50 -10.00 , 1.00 -20.00 , 1.00 -20.0 , 1.00-20.00 , 1.00 -20.00 mL · L-1 , respectively.The coumarins showed the overall recoveries ranging from 96.4%-100.0%.The peaks of determined compounds were identified by standardized reference ex-tract.The contents of coumarins in samples were respectively determined by individual calibration curve method and single compound quantitative evaluation.The results had a good consistence , but the contents of five compounds of Angelica dahurica from different areas had obvious differences.Conclusion The strategy is feasible and credible, which is easily and effectively adapted for the quality control of Angelica dahurica.
