HPLC-MS/MS for the determination of Hydromorphone-3-glucuronidecuronide in human plasma
10.13699/j.cnki.1001-6821.2016.04.014
- VernacularTitle:高效液相色谱－质谱联用法测定人血浆中氢吗啡酮－3－葡萄糖苷酸的质量浓度
- Author:
Xue-Yi CHEN
1
;
Guang-Tao HAO
;
Long-Mei CHENG
;
Ying-Fu ZHANG
;
Rui-Hua DONG
;
Xiao-Fang WANG
;
Yuan-Yuan LI
;
Ze-Yuan LIU
;
Heng-Yan QU
Author Information
1. 军事医学科学院 附属医院 临床药理室
- Keywords:
HPLC-MS/MS;
hydromorphone-3-glucuronide
- From:
The Chinese Journal of Clinical Pharmacology
2016;(4):330-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC -MS/MS method for the determination of hydromorphone -3 -glucuronide in human plasma.Methods The plasma was treated with solid -phase extraction.The column was XTerra?MS C18 column (2.1 mm ×50 mm,5 μm), the mobile phase consisted of 95% acetonitrile -5 mmol · L-1 ammonium bicarbonate with a gradient elution and the flow rate was 0.3 mL· min -1 , column temperature was 20 ℃ with positive ion scan and multiple reaction monitoring mode.The method was validated by investigation of the method specificity ,the standard curve and lower limit of quantification , precision and recovery , matrix effects and stability.Results Hydromorphone -3 -glucuronide had a good linear relationships in the range of 0.5-100.0 ng · mL-1 ( r>0.999 5 ) , the limit of quantification was 0.5 ng · mL-1.Intra -day and inter -day RSD were less than 15%, the average recovery were >60%, and stability was good.Conclusion The method was simple , rapid, sensitive and accurate , suitable for measuring the concentration of hydromorphone -3-glucuronide in human plasma.
