Determination of ciprofloxacin in human serum by high performance liquid chromatography-fluorescence detector
10.13699/j.cnki.1001-6821.2016.03.018
- VernacularTitle:高效液相色谱荧光检测法测定人血清中环丙沙星浓度研究
- Author:
Meng-Jia GE
1
;
Yang LIU
;
Xiao-Guang LI
Author Information
1. 北京大学 第三医院 药剂科
- Keywords:
ciprofloxacin;
HPLC -FLD;
drug concentration;
protein precipitation;
pharmacokinetic
- From:
The Chinese Journal of Clinical Pharmacology
2016;(3):251-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of cipro-floxacin concentration in human serum by high performance liquid chro-matography-fluorescence detector method ( HPLC-FLD).Methods Ciprofloxacin and the internal standard ( IS) gatifloxacin were extracted from human serum after protein precipitation by 5%perchloric acid , then detected with fluorescence detector after excitation at 278 nm after sepa-ration by an Eclipse XDB -C18 (150 mm ×4.6 mm,5 μm).The mobile phase consisted of acetonitrile -phosphate ( pH 3 ) =15∶85 , with a flow rate of 1.0 mL· min-1 using gradient elution .Results Linear calibra-tion curves were obtained in the range of 0.05 -50.00 μg · mL-1 ( r>0.999 5 ).Intra-day RSD were in the ranges of 0.98%-2.63%and inter-day RSD were in the ranges of 1.87%-3.16%.Conclusion The method provides a sensitive , accurate , precise and reliable analy-tical procedure for clinical monitoring of ciprofloxacin in serum.
