Adenoviral short hairpin RNA targeting phosphodiesterase 5 inhibits myocardial cells apoptosis at early stage post-myocardial infarction
10.13699/j.cnki.1001-6821.2016.03.014
- VernacularTitle:靶向磷酸二酯酶5的短发夹 RNA重组腺病毒载体对小鼠急性心梗后早期细胞凋亡的影响
- Author:
Hong CHEN
1
;
Jin-Wen XIAO
;
Long-Hu LI
;
Bing-Zhe HONG
;
Jian-Qi XIAO
;
Dong-Mei WEI
Author Information
1. 齐齐哈尔市第一医院
- Keywords:
short hairpin RNA;
interference therapy;
myocardial infarction;
phosphodiesterase 5
- From:
The Chinese Journal of Clinical Pharmacology
2016;(3):238-240
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of adenoviral short hairpin RNA (shRNA) targeting phosphodiesterase 5 (PDE5 shRNA) on myo-cardial cells apoptosis at early stage post -myocardial infarction ( MI ).Methods MI was induced in mice by left coronary artery ligation.Mice were randomly assigned to test group [ n=10 , adenoviral vectors inserted with shRNA sequence for the inhibition of phosphodiesterase 5 ( PDE5 ) were injected intramyocardially to infarcted and border area ] and control group ( n =10 , injection with adenoviral vectors without therapeutic PDE5 shRNA ).One week post -MI, apoptosis was evaluated by TdT-mediated dUTP nick -end labeling ( TUNEL ) , proteins were extracted from the left ventricular of heart , level of PDE5 expression was detected using Western Bloting , the level of guanosine 3′, 5′-cyclic phosphate ( cGMP) or protein kinase G ( PKG) activity in the left ven-tricular of heart was evaluated by enzyme linked immunosorbent assay (ELISA).Results One week post -MI, compared to control group, test group had reduced number of apoptotic cells in infarct and periinfarct areas, and significantly reduced cardiomyocytes apoptosis in the periinfarct regions ( P<0.05 ).Compared to control group , the level of PDE5 was significantly decreased and the levels of cGMP and PKG were significantly increased in the test group ( P <0.05 ).Conclusion It the PDE5 shRNA has protective effect on acute myocardial infarction and significantly inhibit the apopto -sis of myocardial cell which may be closely related to the increased expression of cGMP and PKG .
