Effect of oxysophocarpineon proliferation and apoptosis of human breast cancer cell
10.13699/j.cnki.1001-6821.2016.02.025
- VernacularTitle:氧化槐果碱对人乳腺癌细胞增殖及凋亡的影响
- Author:
Yuan ZI
1
;
Wen-Jian ZHAO
;
Qing CHENG
;
Hua-Fei DENG
;
Ming XIE
;
Han-Jiang HE
Author Information
1. 湘南学院病理研究所
- Keywords:
oxysophocarpine;
human breast cancer cells;
proliferation;
apoptosis;
caspases-3 protein
- From:
The Chinese Journal of Clinical Pharmacology
2016;(2):174-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of oxysophocarpine ( OSC ) on proliferation and apoptosis of human breast cancer cell ( michigan cancer foundation -7 , MCF -7 ).Method There were divided into groups of final oxysophocarpine concentration of 10 ,20 ,50 , 100,200,400,500 μmol· L-1 and the control group.MTT method was used to analyze the inhibitory effect of on the inhibition of MCF -7 in different concentrations in the experimental groups and control group.Cell apoptosis and cell cycle were detected by flow cytometry.The effect of Caspase-3 concentration was detected by Western blot.Results The proliferation inhibition rate of OSC on MCF -7 cell reached 81.80%, and apoptosis rate 81.67%, showing concentration dependent.Half inhibitory concentration for MCF -7 was 165.31 μmol · L-1.OSC had significant effects on the apoptosis of MCF -7, which could make the number of MCF-7 in G0-G1 gradually increase , and the number of G 2-M phase and S phase decrease gradually.OSC could enhance the expression of Caspases-3 protein in MCF-7 cells dose-dependently.Conclusion OSC may inhibit the proliferation of cells by up -regulating the expression of Caspases-3 and changing the cell cycle of MCF -7, thus inhibiting the proliferation of cells and promoting the apoptosis of MCF -7 cells.
