Determination of unbound antofloxacin in human plasma by high performance liquid chromatography
10.13699/j.cnki.1001-6821.2016.01.021
- VernacularTitle:高效液相色谱法测定人血浆中游离安妥沙星浓度研究
- Author:
Min-Ji WEI
1
;
LüYuan
;
Cai-Yun ZHAO
;
Li-Wei SHI
;
Zi-Sheng KANG
Author Information
1. 北京大学 第一医院 临床药理研究所
- Keywords:
antofloxacin;
unbound concentration;
pharmacokinetic;
plasma
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(1):65-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an analytical method for analyzing the unbound concentration of antofloxacin in human plasma .Methods A HPLC method was developed to determine the unbound concentration of antofloxacin in human plasma using ultra -centrifugation.The column was Alltima C18 (150 mm ×4.6 mm,5μm) with room temperature as the column temperature .The mobile phase was composed of 13:87 acetoni-trile-0.05 mol · L-1 phosphate buffer ( pH 3.0 ) with detection wave-length set at 293 nm, and the flow rate was 1.0 mL · min-1 .The method was validated for specificity , precision and accuracy , recovery as well as stability.Results The calibration curves were linear over the range of 0.08-5.49 μg· mL-1 .The intra-day and inter-day preci-sion evaluated at lower limit of quantification and quality control levels were within 2.78%-10.03%.The recoveries calculated for the anto-floxacin were within 92.09%-108.96%from spiked plasma ultrafiltrate samples.Conclusion The analytical method is quick , precise and accuracy and could be used in the analysis of unbound concentration of antofloxacin in plasma.
