Effects of chrysophanol on ammonia-induced cell swelling and glutamate uptake inhibition in cultured astrocytes and the study of its mechanisms
10.13699/j.cnki.1001-6821.2016.01.019
- VernacularTitle:大黄酚对氨诱导星形胶质细胞肿胀及谷氨酸再摄取抑制的影响及其机制研究
- Author:
Zhan-Xia XUE
1
;
Yong-Shan GAO
;
Li-Xia SHEN
;
Xue-Liang WU
Author Information
1. 河北北方学院 药学系 药理教研室
- Keywords:
chrysophanol;
astrocytes swelling;
oxidative stress/nitration stress;
gultamate uptake inhibition
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(1):59-61,64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of chrysophanol ( Chry ) on ammonia-induced cell swelling and gultamate uptake inhibition in cul-tured astrocytes and its signaling pathways .Methods Primary cultured mouse astrocytes were divided into normal group , model group , three Chry test group ( 0.1 , 1.0 , 10.0 μg · mL-1 ) and six positive control group[L-NAME 250 μmol· L-1, vitamin E 100 μmol· L-1, supero-xide dismutase ( SOD ) 25 U · mL-1 , extracellular regulated kinase 1/2 ( ERK1/2 ) inhibitor UO126 10 μmol · L-1 , P38MAPK inhibitor SB239063 10 μmol · L-1 , JNK1/2/3 inhibitor SP600125 1 μmol· L-1 ].Cultured astrocytes treated for 48 h, cell volume were measured by measuring the volume of water in cell and gultamate uptake were measured by the isotope labeling method after 72 h.The glutamate/aspartate transporter ( GLAST ) mRNA expression was detected by RT-PCR.Results Ammonia-induced astrocytes swelling and gulta-mate uptake inhibition and GLAST mRNA expression downregulation were reduced by moderate and lager doses Chry , which similar to the positive control.Conclusion Chry may improve the ammonia-induced astrocytes dysfunction by againsting oxidative stress and inhibiting theMAPKs phosphorylation.
