Effect of Tetramethylpyrazine in combination with cisplatin on the growth and angiogenesis of transplanted Lewis lung carcinoma in mice
10.13699/j.cnki.1001-6821.2016.01.017
- VernacularTitle:川芎嗪联合顺铂影响Lewis肺癌小鼠移植瘤生长及微血管生成的研究
- Author:
Bu WANG
1
;
Zhi-Hua ZHANG
;
Xiu-Long ZHANG
;
Xin GU
;
Jian-Hua TANG
Author Information
1. 河北北方学院 研究生部
- Keywords:
Tetramethylpyrazine;
Lung carcinoma;
kruppel-like factor 4;
angiogenesis
- From:
The Chinese Journal of Clinical Pharmacology
2016;32(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of Tetrame-thylpyrazine ( TMP ) in combination with cisplatin on the tumor growth and angiogenesis in mouse Lewis lung cancer .Methods Lewis lung carcinoma cells were inoculated subcutaneously into right axillary of C57BL/6 mice, and the mice were randomly divided into 4 groups:model group, TMP group,cisplatin ( DDP) group and combination group ( TMP+DDP).From day 7 after inoculation, all the mice were given different treatments for 14 days, and change of the xenografted tumor volumes was monitored.All mice were sacrificed on day 21.Subcutaneous tumors were processed for tumor weight , and tumor growth inhibition rate and tumor necrosis rate of each group were calculated .The expression level of Kruppel -like factor 4 ( KLF4 ) , vascular endothelial growth factor ( VEGF ) were measured by Western blot . The microvascular density ( MVD) was determined by CD 105 staining.Results The tumor growth was suppressed in TMP group , cisplatin group and combination group .The weights of tumor were markedly decreased in TMP group , cisplatin group and combination group compared with model group ( P<0.05 ) .The combination group showed significant enhancement in anti -tumor efficacy than TMP group and DDP group (62.48%vs 35.58% vs 40.41%,P<0.01).Compared with DDP group,the tumor necrosis rate in combination groupwas significantly higher [ ( 24.64 ±11.96 )% vs ( 31.76 ±15.20 )%, P<0.05 ] , but not remarkably in TMP group [(15.58 ±3.19)%,P>0.05].The expression levels of vascular endothelialgrowth factor and microvessel density were significantly lower ,Kruppel-like factor 4 was significantly higher in TMP group , cisplatin group and combination group than that in model group ( P<0.05 ) .Meanwhile , there was no significant difference between cisplatin group and tetramethylpyrazine group ( P>0.05 ).Conclusion TMP has synergetic inhibitory effect with DDP on the growth and angiogenesis of Lewis lung carcinoma effectively by inhibiting expression of VEGF , enhancing expression of KLF4.
