In vitro activity of colistin combination with biapenem against carbopenems -resistant Pseudomonas aeruginosa
10.13699/j.cnki.1001-6821.2015.24.021
- VernacularTitle:多黏菌素E联合比阿培南对耐碳青霉烯铜绿假单胞菌的体外抗菌活性研究
- Author:
Hui NIU
1
;
Bei-Bei LIANG
;
Xue ZHANG
;
Xiu-Zhen DI
;
Ming-Jing TANG
;
Rui WANG
;
Jin WANG
Author Information
1. 中国人民解放军总医院 药物临床研究中心
- Keywords:
carbapenem -resistant Pseudomonas aeruginosa;
colistin;
biapenem;
fractional inhibitory concentration;
time-killing curve
- From:
The Chinese Journal of Clinical Pharmacology
2015;(24):2437-2439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the antibiotic effects of colistin con-bined with biapenem against 85 isolates of Carbapenems -resistant Pseudomonas aeruginosa( CRPA) in vitro.Methods The minimal inhib-itory concentration ( MIC ) of colistin or biapenem against 85 strains of CRPA was determined by agar dilution method.The broth microdilution and checkerboard method were used to determine the MICs of colistin or biapenem and fractional inhibitory concentration index ( FICI ) was calcu-lated according to MIC results.The time-killing curves of colistin and biapenem alone or combination were determined.Results The MIC of all the combined antimicrobials was reduced significantly (P<0.01).Of 85 isolates, 11.76% synergism, 17.65% section synergism, 34.12%additive effect, 36.47%indifference, no antagonism found.At 24 h the combination resulted in more than 2 log10 CFU? mL-1 reduction in viable bacteria compared with colistin or biapenem alone.Conclusion The activities of colistin combined with biapenem are mainly synergistic and additive.
