Simultaneous determination of six coumarins in Radix Glehniae using pressurized liquid extraction and HPLC
10.13699/j.cnki.1001-6821.2015.24.020
- VernacularTitle:加压溶剂提取－HPLC法测定北沙参中6种香豆素类化合物
- Author:
De-Qiang LI
1
;
Yuan-Yuan XIAO
;
Rong-Yuan DAI
;
Ai-Lian SHAN
Author Information
1. 河北医科大学 第二医院 药学部
- Keywords:
pressurized liquid extraction;
HPLC;
Radix Glehniae;
coumarin
- From:
The Chinese Journal of Clinical Pharmacology
2015;(24):2433-2436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for simultaneous de-termination of six coumarins in Radix Glehniae from different sources. Methods The samples were analyzed by HPLC after pressurized liquid extraction ( PLE ) .The chromatographic conditions were as following:The column was Kinetex core-shell-C18 column (50 mm ×4.6 mm, 2.6 μm) , the mobile phase consisted of acetonitrile-water using gradi-ent elution, the flow rate was 0.4 mL? min-1 and the column tempera-ture was 30℃.The detection wavelength was set at 310 nm and the sam-ple injection volume was 20 μL.The method was validated by investiga-ting its specificity, linearities and limit of detection, precision, extraction recovery, stability and repeatability.Results Six components were de-termined by HPLC, including psoralen, xanthotoxin, bergapten, impera-torin, cnidilin and isoimperatorin. The linearities of them were good ( r≥0.999 2 ) in the concentration ranges of 0.50 -10.00 , 1.00-20.00, 0.32-6.40, 0.25 -5.00, 0.05 -1.00, 0.17 -3.50μg? mL-1 , respectively.The coumarins showed the overall recoveries ranging from 91.7%-107.6%.The contents of six compounds of Radix Glehniae from different areas had an obvious difference.Conclusion Six coumarins in Radix Glehniae were analyzed by PLE and HPLC. The method has good specificity, reproducibility and high sensitivity, which can be applied for the quality control of Radix Glehniae.
