Effects of sulindac sulfide on proliferation and apoptosis of SKBR-3 cells
10.13699/j.cnki.1001-6821.2015.23.016
- VernacularTitle:舒林酸硫化物对人乳腺癌细胞 SKBR－3增殖与凋亡的影响
- Author:
Yun-Jiang ZHOU
1
;
Hu WANG
;
He-Huan SUI
;
Li LI
;
Jia-Jun HUANG
Author Information
1. 重庆医科大学 药学院 药理学教研室 重庆市生物化学与分子药理学重点实验室
- Keywords:
sulindac sulfide;
SKBR-3 cell;
proliferation;
apoptosis;
Bcl-2;
Bax;
Caspase-3
- From:
The Chinese Journal of Clinical Pharmacology
2015;(23):2325-2328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sulindac sulfide on proliferation and apoptosis of SKBR-3 cells, and then explore its poten-tial molecular mechanism.Methods SKBR -3 cells were incubated with different concentrations of sulindac sulfide (20, 40, 80 μmol? L-1 ) for 24, 48, 72 h, normal group and control group were respectively trea-ted with medium and 0.1%DMSO for the same time.Apoptosis and pro-liferation of SKBR-3 cells were analyzed by using flow cytometry and MTT assay, respectively.Western blot was used to test the protein ex-pression of Bcl -2, Bax, cytochrome C, Caspase -3 and Cleaved Caspase -3. Results Sulindac sulfide inhibited the proliferation of SKBR-3 cells and promoted the apoptosis of cells.After 24 hours of in-cubation, the protein expression of Bcl-2 and mitochondrial cytochrome C markedly reduced ( P<0.05) , the protein expression of Bax, cytosol-ic cytochrome C and Cleaved Caspase -3 significantly increased ( P<0.05) , the protein expression of Caspase -3 had no significant difference (P>0.05). Conclusion Sulindac sulfide significantly inhibit the proliferation of SKBR-3 cells and promote cells apoptosis. The potential molecular mechanism of pro -apoptosis might be that sulindac sulfide down -regulates the protein expression of Bcl -2 , up-regulates the protein expression of Bax, induces the release of cytochrome c from mitochondria, finally activates the Caspase-3 protein and then promotes the apoptosis of SKBR-3 cells.
