Determination of oxcarbazepine and its metabolite 10 , 11-dihydro-10-hydroxy carbamazepine in human plasma by solid phase extraction and HPLC
10.13699/j.cnki.1001-6821.2015.22.025
- VernacularTitle:固相萃取-高效液相色谱法测定人血浆中奥卡西平及其代谢产物10-羟基卡马西平的浓度
- Author:
Wan-Cai QUE
1
;
Hong-Qiang QIU
;
Yu CHENG
;
Zhi-Qiang ZHANG
;
Zhao-Yang WU
;
Jia YANG
;
Mao-Bai LIU
Author Information
1. 福建医科大学 附属协和医院 药学部
- Keywords:
oxcarbazepine;
10;
11-Dihydro-10-hydroxy carbamazepine;
solid phase extraction;
HPLC
- From:
The Chinese Journal of Clinical Pharmacology
2015;31(22):2254-2256,2258
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for the determination of ox-carbazepine (OXC) and 10,11-dihydro-10-hydroxy carbamazepine (MHD) in human plasma by solid phase extraction ( SPE) and high-performance liquid chromatography ( HPLC) .Methods OXC and MHD in human plasma was extracted by SPE tubes.The OXC and MHD were determined by HPLC.The chromatographic conditions were as following:the column was Shim-Pack C18 (250 mm ×4.6 mm,5μm ) , the mobile phase consisted of methanol and water (44:56 ) with a flow rate of 1.0 mL·min -1 , the injection volume was 40 μL, temperature was 25 ℃, detective wavelength was set 240 nm.Results OXC and MHD were in good linearity between 0.05-20 μg·mL-1 and 0.05-20 μg·mL-1 , respectively. Limit of detection for OXC and MHD were 25 , 50 ng·mL-1 ( S/N≥3 ) , respectively.Average recovery of extraction for OXC and MHD were 87.9%and 88.1%, respectively.Average method of extraction for OXC and MHD were 98.2%and 96.7%, respectively. Average between-day RSD for OXC and MHD were 6.5%and 5.9%as well as 4.8%and 4.0%of within-day RSD, respectively.Conclusion The established method for determination of OXC and MHD in human plasma by SPE is suitable for clinical application with high sensitivity, simple operation, high accuracy and reliability.
