Inhibition of Gekko ethanol extract on the proliferation of C6 glioma cells
10.13699/j.cnki.1001-6821.2015.22.023
- VernacularTitle:壁虎醇提物抑制C6胶质瘤细胞增殖作用的研究
- Author:
Wen-Jing GE
1
;
Jian-Gang WANG
;
Ying JIN
;
Rui-Fang LI
;
Leng-Xin DUAN
;
Ling LIU
Author Information
1. 河南科技大学 医学院 药理学与分子生物学实验室
- Keywords:
C6 cell;
Gekko ethanol extract;
apoptosis;
caspase-9;
ap-optosis inducing factor
- From:
The Chinese Journal of Clinical Pharmacology
2015;31(22):2249-2251
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of Gekko etha-nol extract ( GEE) on the proliferation of C6 glioma cells and explore the possible molecular mechanism. Methods Groups were divided into blank group, control group (0.01 mg·mL-1 5-fluorouracil) and ex-perimental groups (0.1, 0.15, 0.2, 0.3, 0.4 mg·mL-1 GEE).The inhibitory effect of GEE on C6 cells were detected by MTT assay.C6 cells were cultured with GEE for 24 h.The morphologic changes of C6 cells were observed with inverted microscope.The nucleus morphological changes were observed by Hoechst 33258 fluorescence staining.The ex-pression of cysteinyl aspartate specific proteinase-9 ( caspase-9 ) and apoptosis inducing factor ( AIF ) were detected by Western blot assay. Results GEE significantly inhibited proliferation of C6 cells after 48 h of treatment. The inhibitory rates of five experimental groups were 19.9%, 28.7%, 63.1%, 75.4%, 76.3%, respectively, and there were significant difference compared to that of blank group ( P<0.05 ) . The typical morphological changes of apoptosis were observed in treated C6 cells.Result of Western blot showed that GEE induced up-regula-tion of caspase-9 and AIF.Conclusion GEE can inhibit the prolifera-tion and induce apoptosis of C6 cells, which may be associated with the increasing expression of caspase-9 and AIF.
