Influences of deduhonghua -7 powder on transforming growth factor beta and smad protein pathways in liver fibrosis tissue
10.13699/j.cnki.1001-6821.2015.22.022
- VernacularTitle:德都红花-7味散对肝纤维化组织中转化生长因子β1及smad蛋白通路的影响
- Author:
Yan-Hua XU
1
;
Zhi-Qiang HAN
;
Dan SHAN
;
Na TA
;
Ri-Su NA
;
Da AN
Author Information
1. 内蒙古民族大学 附属医院 蒙药临床药理研究所
- Keywords:
deduhonghua-7 powder;
liver fibrosis;
transforming growth factor beta 1;
smad protein research
- From:
The Chinese Journal of Clinical Pharmacology
2015;31(22):2246-2248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic effect of deduhonghua-7 powder on liver fibrosis and explore its relevance with transforming growth factor beta ( TGF-β) and smad protein pathways.Methods The liver fibrosis rat model were randomly divided to A group, B group, C group, D group, 8 rats for each.A group was administered 0.6 g·kg-1 of deduhonghua -7 powder, B group was administered 0.4 mg·kg-1 of colchicines, C group and D group were administered dis-tilled water intragastrically 1 time per day for 40 days.The liver tissue was prepared by histopathology slides, than elastic and collagen fiber staining was acted to observe the degree of fibrosis.Enzyme-linked im-munosorbent assay ( ELISA ) was acted to detect the hyaluronic acid ( HA) , laminin ( LN) content of liver tissue homogenate.Immunohisto-chemistry detecting was acted to determine the changes of smad2, smad3, smad6, smad7, and TGF-β1 protein expressions.Results Compared with D group, the collagen fiber and contents of HA, LN of C group significantly increased (P<0.05).Compared with C group, the collagen fiber and the contents of HA, LA of A group and B group significantly decreased (P<0.05).The TGF-β1, smad2, smad3 protein expressions of C group were up-regulated compared with those of D group with significant differences ( P<0.05).The TGF-β1, smad2, smad3 protein expres-sions of A and B groups were down-regulated compared with those of C group, with significant differences ( P <0.05) .The smad6, smad7 protein expressions of C group were down-regulated compared with those of D group, with significant differences (P<0.05).The smad6, smad7 protein expressions of A and B groups were up-regulated com-pared with C group, with significant differences (P<0.05).Conclusion Deduhonghua-7 powder can regulate the TGF-β/smad protein patheways and block the TGF-βexpression, inhibit the synthesis of extracellular matrixc and degradation of extracellular matrixc to reach the purpose of blocking and deferring the development of liver fibrosis.
