Advanced development of target molecule affinity coupled with mass spectrometry for the screening of bioactive compounds in traditional Chinese medicines
10.13699/j.cnki.1001-6821.2015.21.025
- VernacularTitle:靶分子亲和－质谱联用技术在中药活性成分筛选中的应用进展
- Author:
De-Qiang LI
1
;
Yuan-Yuan XIAO
;
Rong-Yuan DAI
;
Zhi-Qing ZHANG
;
Ai-Lian SHAN
Author Information
1. 河北医科大学 第二医院 药学部
- Keywords:
bioactive compound;
screening;
traditional Chinese medi-cines;
target;
target molecule affinity coupled with mass spectrometry
- From:
The Chinese Journal of Clinical Pharmacology
2015;(21):2164-2169
- CountryChina
- Language:Chinese
-
Abstract:
Rapid screening and characterization of bioactive compounds from traditional Chinese medicines ( TCM) is one of the highlighted re-search challenges in the field of drug development.Target molecule affin-ity coupled with mass spectrometry ( MS) has become a new method for the screening of bioactive compounds from complex mixture, the recent development of which since 2005 will be reviewed.According to the prin-ciple of affinity screening, the methods could be classified into pre -column affinity screening including ultrafiltration -MS, size -exclusion chromatography-MS, turbulent-flow chromatography-MS, restricted-access material-MS and immobilized target-MS and post-column af-finity screening (HPLC-continuous-flow bioassay-MS).Each meth-od with its typical applications would be introduced, providing technique references for the screening of bioactive compounds from complex mixture such as TCM.
