Determination of nilotinib in human plasma by UPLC-MS/MS

VernacularTitle:超高效液相色谱－串联质谱法测定人血浆中尼洛替尼的研究
Author: You-Bin PU ¹ ; Ji DONG ; Li-Yan MIAO
Author Information

1. 苏州大学附属第一医院药学部
Keywords: nilotinib; UPLC -MS/MS; therapeutic drug monitor; plasma
From: The Chinese Journal of Clinical Pharmacology 2015;(21):2143-2145
CountryChina
Language:Chinese
Abstract: Objective To establish a UPLC-MS/MS method for the determination of nilotinib in human plasma.Methods The plasma procedure involved a single-step protein precipitation by acetonitrile, then separation was carried out using a ACQUITY UPLC BEH C18 column ( 100 mm ×2.1 mm, 1.7 μm) with a mobile phase consisted of acetonitrile -0.1% formic acid ( 35∶65 ) at a flow rate of 0.4 mL? min -1 .The protonated ions of analytes were detected in positive ionization by multiple reaction monitoring mode ( MRM ) .The speci-ficity, standard curve, lower limit of quantitation, precision, recovery rate and stability as well as the matrix effect were investigated. Results The calibration curve was linear over a concentration range of 0.1 to 20.0 μg? mL -1 ( r=0.998) and the low limit of quantita-tion was 0.1 μg? mL -1 .The extraction recovery rates were ranged from 95.11% to 101.32%, with-day RSD and between-day RSD were all less than 15%.Conclusion The method is simple and quick, with high specificity and sensitivity, and suitable for clinical therapeutic drug monitoring of nilotinib.