Simultaneous determination of rifampicin, rifapentine and rifabutine in child plasma by UPLC-MS/MS
10.13699/j.cnki.1001-6821.2015.21.015
- VernacularTitle:超高效液相色谱质谱联用法测定儿童血浆中利福平、利福喷丁和利福布汀的浓度研究
- Author:
Jing AN
1
;
Hong-Tao LIU
;
Zhan-Jun DONG
;
Xiao-Dan ZHANG
Author Information
1. 河北省人民医院药学部
- Keywords:
rifampicin;
rifapentine;
rifabutine;
UPLC-MS/MS;
blood concentration
- From:
The Chinese Journal of Clinical Pharmacology
2015;(21):2134-2136
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the simultaneous deter-mination of rifampicin, rifapentine and rifabutine in child plasma by UPLC-MS/MS.Methods Zaleplon was used as internal standard. The proteins of plasma samples were precipitated with acetonitrile.The fluid was separated on the ACQUITY UPLC HSS T3 column ( 2.1 mm × 100 mm,1.8 μm) .The mobile phase consisted of acetonitrile and 15 mol? L-1 ammonium formate -0.05% formic acid solution in gradient elution.Electrospray ionization ( ESI) source was applied and operated in the positive multiple reaction monitoring ( MRM) mode.The specificity, standard curve and lower limit of quantitation, precision and recovery rate and stability as well as the matrix effect were investigated.Results The linear ranges of rifampicin, rifapentine, rifabutine were 100-5000, 20-2000, 20-2000 ng? mL-1 , respectively.The intra-day and inter-day precision ( RSDs) were less than 15%.Conclusion The method is sensi-tive, simple and accurate.The therapeutic drug monitoring of three antitu-berculosis drugs could be performed at the same time.
