Similarity analysis of Sinisan lyophilized power in serum by HPLC fingerprint

VernacularTitle:HPLC指纹图谱分析血清中四逆散冻干粉的相似度
Author: Yue-Feng LI ¹ ; Rui CAO ; Fu-Ju XU ; Ze-Guo ZHANG ; Jiang-Tao NIU ; Xing-Ke YAN
Author Information

1. 甘肃中医药大学药学院中药炮制教研室
Keywords: Sinisan lyophilized power; serum; HPLC; fingerprinting
From: The Chinese Journal of Clinical Pharmacology 2015;(21):2131-2133
CountryChina
Language:Chinese
Abstract: Objective To establish a sensitive and specific high per-formance liquid chromatography ( HPLC ) method for similarity analysis of Sinisan lyophilized power in rats serum.Methods HPLC method was applied.The chromatographic conditions were as following: Column:Kromasil C18 column ( 4.6 mm ×250 mm, 5 μm );flow -rate:1.0 mL? min -1;column temperature:30 ℃.The mobile phase:acetonitrile ( A) and H2 O ( acidified to pH=3 with phosphoric acid) ( B) with a gra-dient elution.The concentration of solvent A was from 10% to 90% in 150 min.Ten batches Sinisan lyophilized powder samples in serum were analyzed by HPLC fingerprint.Results The HPLC chromatographic fingerprin-ting of Sinisan showed 8 characteristic peaks compared with that of blank serum, they were absorbed into blood.The result of sim-ilarity analysis was over 0.99. Conclusion The chromatographic fingerprinting of Sinisan lyophizied power had high specificity and characteristic.