Effect of down regulation of EZH2 expression by shRNA on the apoptosis and proliferation of human colorectal cancer cell
10.13699/j.cnki.1001-6821.2015.21.012
- VernacularTitle:shRNA下调 EZH2基因表达对人结肠癌细胞凋亡、增殖的影响
- Author:
Kun-Hou YAO
1
;
Lun-Shou WEI
;
Wan-Li MA
;
Jun-Hong HU
Author Information
1. 河南大学 淮河医院 普通外科
- Keywords:
colorectal cancer;
gene of EZH2;
small interfering RNA;
apoptosis;
proliferation
- From:
The Chinese Journal of Clinical Pharmacology
2015;(21):2125-2127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of down regulation of EZH2 expression by shRNA on apoptosis and proliferation in colorectal cancer cell.Methods The human colorectal cancer cell line HCT116 was used as the materials.The EZH2 expression was knockdown by traninfecting siRNA.The EZH2 protein expression was tested by western-blot array, the HCT116 apoptosis rate was tested by flow cytometry array and the HCT116 cell proliferation ability was tested by MTT array.Results The EZH2 protein significant decreased by si -EZH2 ( P <0.05 ) .The apoptosis rate significant increased by knockdown the EZH2 expression ( P<0.05) .And the proliferation ability was inhibited after knockdown the EZH2 expression ( P<0.05).Conclusion EZH2 may be involved in the process of apoptosis and proliferation in human colorectal cancer cells which could be a potential target for treatment.
