Protective effects of hypothermia union Shengmai and dexamethasone on the spermatogenic cell apoptosis of torsioned testes in rats
10.13699/j.cnki.1001-6821.2015.20.017
- VernacularTitle:局部低温联合生脉与地塞米松对睾丸扭转复位后生精细胞凋亡的保护作用
- Author:
Jia-Bin ZHANG
1
;
Guang-Bing CHEN
;
Wu-Bin WENG
;
Chun ZHANG
Author Information
1. 福建医科大学 附属闽东医院 泌尿外科、宁德市泌尿外科研究所
- Keywords:
testicular torsion;
hypothermia;
Shengmai;
dexamethasone;
spermatogenic cell apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2015;(20):2037-2039
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of hypothermia union Shengmai and dexamethasone on spermatogenic cell apoptosis of torsioned testes in rats.Methods Forty-eight adolescence male Spra-gue-Dawley rats were random divided into 6 groups.Each group of 8 rats, six group rats were left testicular torsion 720°for 2 h, establish a u-nilateral testicular torsion model.Group A:normal temperature+saline;group B:normal+Shengmai+dexamethasone; group C:low temperature+saline;group D:low temperature+dexamethasone; group E:low tem-perature+Shengmai;group F:Shengmai+dexamethasone+low tempera-ture.The testicles were collected after 48 h, the testicular histopathologi-cal changes were observed by HE staining light microscopy.The apopto-tic spermatogenic cells law were detected by TdT-mediated dUTP nick-end labeling ( TUNEL) .Results Group B, C, D and E testicular tissue pathology damage visible parts of pure cell falls off born.The born small tube of testicular sperm cells in group F is born slightly off, general structure and tidy.Apoptosis index ( AI ) in group A ( 35.13 ±2.21 ) was higher than that group B ( 18.12 ±5.23 ) , group C ( 17.13 ±3.03 ) , group D ( 17.05 ±1.03 ) , group E ( 16.39 ±2.02;all P<0.05 ) and group F ( 9.06 ±1.53;P <0.01 ) . Conclusion Testicular torsion reduction ischemia-reperfusion injury can lead to life essence cell apoptosis increase.Hypothermia union Shengmai and dexa-methasone on spermatogenic cell apoptosis of torsioned testes in rats have good protection.
