Efficacy evaluation of saikosaponin d in treatment of hepatoma model rat in vivo
10.13699/j.cnki.1001-6821.2015.20.014
- VernacularTitle:柴胡皂苷 d在肝癌模型大鼠体内的药效学评价
- Author:
Wei WANG
1
;
Hai-Lin LI
;
Guang-Ning HAN
;
Guo-Quan SONG
;
Hai-Ping DAI
;
Yong-Hai PENG
Author Information
1. 牡丹江医学院 红旗医院 普外二科
- Keywords:
saikosaponin d;
hepatocellular carcinoma;
proliferating cell nuclear antigen;
MKI -67 gene encodes nuclear protein
- From:
The Chinese Journal of Clinical Pharmacology
2015;(20):2027-2030
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the pharmacodynamics of saikosapo-nin d ( SSd) in the treatment of hepatoma model rat.Methods Sixty SD rats with succeed modeling were randomly divided into model group ( n=20 ) , experimental group ( n=20 ) and control group ( n=20 ) , 20 SD rats without modeling were conducted to placebo group.The experi-mental group was treated with SSd 10 mg? kg -1 once a day, the control group was treated with thalidomide 200 mg? kg -1 once a day, the place-bo group and model group were treated with 0.9% NaCI 2 mL once a day.Liver function index were compared by Biochemical analysis me-thod, proliferating cell nuclear antigen ( PCNA) and MKI-67 gene en-codes nuclear protein(Ki-67) were compared by immunohistochemistry after 6 weeks.Results Compared with the model group, liver function index as well as the expression of PCNA and Ki-67 of the control group and the experiment group were lower (P<0.05).Compared with the control group, liver function index with expression of PCNA and Ki-67 of the experiment group were lower ( P<0.05).Pathological display:the pathological sections of the liver in the placebo group showed that the structure of the normal liver was complete, the liver cells were neat, the liver cells were arranged regularly, and the nu-cleus was clear.The model group showed that the tumor foci were in the shape of the necrosis, and the structure of the normal liver was destroyed, and the white nodules were the liver cancer cells.A small amount of low malignant degree, focal necrosis of cancer cells and fewer mitoses could be observed in control group, a small amount of blood and rare mitotic figures could be seen in the experiment group.Conclusion SSd can significantly improve liver function, repair the damage of liver cells, and reduce protein expression of PCNA and Ki-67 in model rats.
