Simultaneous determination of the 6 kinds of aconitum alkaloid contents in Mongolian medicine Garidi-13 pill by HPLC method
10.13699/j.cnki.1001-6821.2015.19.023
- VernacularTitle:HPLC法测定蒙药嘎日迪-13味丸中6种乌头类生物碱的含量
- Author:
BATU-Deligen
1
;
BUREN-Batu
;
Zhi-Qiang HAN
Author Information
1. 内蒙古民族大学附属医院蒙药临床药理研究所
- Keywords:
Mongolian medicine Garidi -13 pill;
HPLC;
aconitine;
hypaconitine;
mesaconitine;
benzoylaconitine;
benzoylhypacoitine;
benzoylmesaconine
- From:
The Chinese Journal of Clinical Pharmacology
2015;31(19):1961-1963
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for simultaneous determi-nation of 6 alkaloid in Mongolia medicine Garidi -13 pill.Methods Diamonsi I C18 column was used.The acetonitrile-tetrahydrofuran (25:15 ) and 0.1 mol? L-1 of ammonium acetate solution were used as mobile phase by gradient elution.The volume flow rate was 0.8 mL? min -1 , and column temperature was 30 ℃, wavelength was 235 nm.The speci-ficity, lower limit of quantification and standard curve , precision and re-covery rate and stability as well as the matrix effect were investigated . Results The aconitine , hypaconitine , mesaconitine , benzoylaconitine , benzoylhypacoitine and benzoylmesaconine showed a good linear relation-ship in the 3.48 ×10 -3 -0.17 ( r=0.999 5 ) ,3.24 ×10 -3 -0.16 ( r=0.999 6 ) ,6.96 ×10 -3 -0.35 ( r=0.999 5 ) ,2.06 ×10 -3 -0.10 ( r=0.999 9 ) , 2.02 ×10 -3 -0.20 ( r=0.999 9 ) and 6.66 ×10 -3 -0.33 ( r=0.999 9 ) mg? mL-1 .The RSD was 3.56%, 1.87%, 4.86%, 2.74% and 3.21%, 4.59%, respectively , and the average recovery rate was 101.11%, 98.07%, 97.81%, 9 9.24%, 104.55% and 104.84%.Conclusion The method is simple , accurate and reproduci-ble, which can simultaneously determinate the mono ester alkaloid and double ester alkaloid in the Mongolia medicine Garidi -13 pills.
