Establishment and international evaluation of drug-dependent platelet antibodies testing
10.13699/j.cnki.1001-6821.2015.19.022
- VernacularTitle:药物(奎宁)依赖性血小板抗体检测技术的建立及国际合作评估
- Author:
Zhou-Lin ZHONG
1
;
Yan ZHOU
;
Jin-Lian LIU
;
Li-Lan LI
;
Wei-Dong SHEN
;
Guo-Guang WU
Author Information
1. 南宁输血医学研究所
- Keywords:
drug-dependent antibodies;
drug-induced immune throm-bocytopenia;
flow cytometric assay for drug -dependent platelet antibo-dies;
monoclonal antibody -specific immobilization of platelet antigens assay for drug-dependent platelet antibodies
- From:
The Chinese Journal of Clinical Pharmacology
2015;31(19):1958-1960
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish drug-dependent antibodies ( DDAb) testing methods , and to organize international labs for evaluation of the methods.Methods Flow cytometric assay for drug -dependent platelet antibodies ( FCM-DDAb) and monoclonal antibody -specific immobi-lization of platelet antigens assay for drug -dependent platelet antibodies ( MAIPA-DDAb ) were established for DDAb testing .Serum samples containing quinine -dependent antibodies , normal serum sample , mAbs, quinine , FCM-DDAb and MAIPA-DDAb protocols were sent to inter-national collaborators who were asked to test the samples with the proto-cols provided.The test results from collaborators were evaluated .Results For the test of three samples , the accuracy of FCM -DDAb test re-sults was 91.67%, 100%and 91.67%, respectively , while the accura-cy of MAIPA -DDAb test results was 100%. Both of DDAb testing methods show good sensitivity and specificity on drug -dependent platelet antibodies testing.Conclusion It can be used for laboratory diagnosis of drug-induced immune thrombocytopenia .
