Role of triptolide/tripterine in glioma cell proliferation induced by connective tissue growth factor
10.13699/j.cnki.1001-6821.2015.19.020
- VernacularTitle:雷公藤单体抑制结缔组织生长因子对脑胶质瘤细胞增殖的影响
- Author:
Chun-Yang MA
1
;
Xiao-Ping WAN
;
Kun YANG
;
Zi-Ling WANG
;
Chuan-Yi ZHENG
;
Fu-Yue YE
;
Ran WU
Author Information
1. 中南大学湘雅医院 肝胆肠外科
- Keywords:
triptolide/tripterine;
glioma cell;
connective tissue growth factor;
Rho-kinase
- From:
The Chinese Journal of Clinical Pharmacology
2015;31(19):1952-1954
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of Rho-kinase in glioma cell proliferation induced by connective tissue growth factor ( CTGF ) and effects of triptolide/tripterine.Methods A subculture growth of glioma cells were got to take the experiment, the normal control group was not give any drug intervention .CTGF group was given CTGF 2.5 μg? L-1 and incubation for 24 h.Triptolide group was given CTGF 2.5 μg? L-1 and triptolide 0.5 ng? mL-1 and incubation for 24 h.Tripterine group were given CTGF 2.5 μg? L-1 and tripterine 0.5 ng? mL-1 and incuba-tion for 24 h.Y27632 ( group specificity of Rho kinase inhibitor ) cells after 30 min to give CTGF 2.5 μg? L -1 and Y27632 1 μmol? L-1 pre-treatment.The expressions of Rho -kinase protein were detected by ELISA.Glioma cell proliferation was measured by 3 H -TdR assay. Results CTGF could induce the proliferation of glioma cell .Triptolide, tripterine and Y27632 ( Rho kinase inhibitor ) could significantly reverse these effects ( P<0.05 ).In addition, CTGF could induce the activation of Rho-kinase ( P<0.01 ) , while triptolide and tripterine could significantly reverse these effects ( P<0.05 ) , indica-ting the activation of Rho -kinase pathway participates in glioma cell proliferation induced by CTGF . Conclusion CTGF could induce the proliferation of glioma cell , while triptolide , tripterine and Y27632 could significantly reverse these effects.Rho-kinase pathway participates in glioma cell proliferation induced by CTGF .
