Determination of free amino acid in serum and urine of patients with diabetes by HPLC
10.13699/j.cnki.1001-6821.2015.18.022
- VernacularTitle:HPLC法测定糖尿病患者血浆和尿中氨基酸含量
- Author:
Xiao-Li MA
1
;
Lei MENG
;
Lin-Lin LI
;
Xin-Xia LI
;
Xin-Min MAO
Author Information
1. 新疆医科大学
- Keywords:
pre -column;
HPLC;
phenyl isothiocyanate;
plasma;
urine;
free amino acid
- From:
The Chinese Journal of Clinical Pharmacology
2015;(18):1869-1872
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a pre -column derivatization and HPLC method for the determination of free amino acids in serum and urine with phenyl isothiocyanate ( PITC ) as derivatization reagent . Methods The determination was performed on waters e 2695 sys-tem.Sample was separated by Shim -pack VP-ODS column(250 mm × 4.6 mm,5 μm ) with a gradient elution of mobile phases composed of methanol/acetate/H2 O( pH 6.5 ) , the content of amino acid were tested by UV at 254 nm.Results The developed method had a good linear ( r>0.991 8 ) in the content range of 2-200 μg· mL-1 , with an aver-age recovery ranged from 88.5% to 123.4%.The precision was within 0.12%-3.42%.Conclusion PITC pre-column derivatization and HPLC method was developed and validated , which had good accuracy , suitable for the determination amino acid in serum and urine of patients with diabetes .
