Effection of total paeony glycosides on the expression of multidrug resistance gene in non-small cell lung cancer model rats
10.13699/j.cnki.1001-6821.2015.18.017
- VernacularTitle:赤芍总苷对非小细胞肺癌模型大鼠耐药基因表达影响
- Author:
Yi-Kun CHEN
1
;
Rong FAN
;
Mei HUANG
Author Information
1. 贵阳护理职业学院 卫生管理系
- Keywords:
total paeony glycoside;
non-small cell lung cancer;
multidrug resistance gene 1;
multidrug-associated protein;
lung resistance protein
- From:
The Chinese Journal of Clinical Pharmacology
2015;(18):1852-1854,1862
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effection of total paeony glycosides on the expression of multidrug resistance gene in non -small cell lung cancer model rats.Methods Fifty male selected rats were randomly di-vided into control group ( 5 rats and only inject saline ) and model group (45 rats).The model group included squamous cell carcinoma , adeno-carcinoma and large cell carcinoma cell line , and each model contained 15 rats, when the models successfully constructed , the rats were grouped into model group , total paeony glycoside group and cyclophosphamide group.The total paeony glycoside group was injected the drug everyday through dorsal vein 240 mg · kg -1 , the model group was given intrave-nous injection of saline 1 mL and cyclophosphamide group was 0.4 mL· kg-1 for 7 days.After experiments , all the rats were executed and the vein blood and lung tissue were sampled for marking tumors , blood testing , histological section and detecting the expression levels of multidrug resistance gene 1-mRNA(MRP-mRNA), lung resistance protein -mRNA(LRP-mRNA) and multidrug resistance associated protein -mRNA ( MDR1 -mRNA ) gene by RT -PCR.Results Compared with the control group, carcino-embryonic antigen(CEA), CA50 level, white blood cell(WBC), and the minimum value of WBC all decreased to some extent in the total paeony glycoside group and cyclophosphamide group of these , the value of CEA , tumor marker 50(CA50) in total paeony glycoside group was significantly lower than values in cyclophosphamide group (P<0.05).While the minimum value of WBC was the opposite (P<0.05).Drug resistant gene in total paeony gly-coside group and cyclophosphamide group were all expressed to varying degrees , but polymerase chain reaction (PCR) product density , MDR1, MRP, and LRP gene expression in the former group all increased and larger than later group (P<0.05).Conclusion Total paeony glycoside can obviously inhibit the diffusion of tumor cells , the expression of lung resistant protein and multi -drug resistant gene , thereby enhancing the resistance and sensitivity of body to drug-sithas instructional significance for clinic .
