Study mechanism induced by exogenous hydrogen sulfide on promotes and stimulates the angiogenesis of rat glioblastoma
10.13699/j.cnki.1001-6821.2015.18.016
- VernacularTitle:外源性硫化氢诱导大鼠神经胶质瘤血管形成机制的研究
- Author:
Shun-Wu XIAO
1
;
Xue-Jun ZHANG
;
Yin DAI
;
Chun-Yue YOU
;
Min-Xiang XIE
;
Pei WANG
;
Yu-Yu WANG
;
Yi LI
Author Information
1. 遵义医学院 附属医院 神经外科
- Keywords:
H2 S;
glioblastoma;
cystathionine -beta -synthase;
3-mercaptopyruvate sulfurtransferase;
matrix metallo proteinase-9;
hypoxia-inducible transcription factors -1α
- From:
The Chinese Journal of Clinical Pharmacology
2015;(18):1849-1851
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism induced by exogenous H2 S on rat glioma formation.Methods Fourty adult SD rats were ran-domly divided into control group , NaHS group , tumor group , tumor +NaHS group of four groups , each group 10.Tumor group , tumor+NaHS group injected rat striatum C 6 glioma cells to established glioma model . Control group, NaHS group received no treatment.Four groups of rats fed normally , three weeks after taking the brain sensitive sulfur electrode applied to detect the rat brain tumor and H 2 S content of its decapitated . The cystathionine -beta-synthase ( CBS ) , mRNA transcription level of 3-mercaptopyruvate sulfurtransferase (3MST) were determined by real time PCR assay. The hypoxia -inducible transcription factors -1α(HIF-1α), vascular endothelial growth factor (VEGF) and the expre-ssion of matrix metallo proteinase-9 ( MMP-9 ) by ELISA and Western blot.The intratumoral micro vessel density ( MVD ) was counted by CD105 -MVD. Results Compared with the control group , NaHS group, tumor group, tumor +NaHS group were clearly visible tumor tissue, significantly increased the number of tumor capillaries .Tumor+NaHS group has more CBS , 3MST, HIF-α, VEGF expression of MMP -9.And tumor +NaHS group was significantly higher than the NaHS group , tumor group ( P<0.01 ) .Conclusion Exogenous H 2 S may induce and promote angiogenesis in rat glioma within its mechanism of H2S promote CBS, 3MST, closely related to the HIF-αVEGF expression of matrix metalloproteinase MMP -9.
