Study on establishment of animal model with diabetes complicated fatty liver induced by streptozocin and nicotinamide
10.13699/j.cnki.1001-6821.2015.18.015
- VernacularTitle:2型糖尿病合并脂肪肝动物模型制备方法研究
- Author:
Dong YAN
1
;
Si-Yi LI
;
Yuan-Yuan WEI
Author Information
1. 新疆医科大学 基础医学院
- Keywords:
diabetes complicated fatty liver;
nicotinamide;
streptozoto-cin;
animal model
- From:
The Chinese Journal of Clinical Pharmacology
2015;(18):1846-1848
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore establishment method of ideal rats model with diabetes complicated with fatty liver .Methods SD rats were induced by high-fat, streptozocin and nicoti-namide.The animal model with diabetes complicated fatty liver induced by streptozocin and nicoti-namide was established.Eight weeks later , the serum of total cholesterol (TC), triglyceride(TG), glutamic-pyruvic transaminase (ALT), glu-tamic oxalacetic transaminase ( AST ) , fasting glucose ( FPG ) , fasting in-sulin ( FINS ) were measured. And the pathological changes of liver tissues were observed.Results There were markedly changes in blood glucose ,blood lipid and transaminases and similar histopathology to fatty liver in rats with STZ 50 mg · kg -1 and high -fat.Compared with the other groups , this group is more typical and more consistent with diabetes and fatty liver pathologic changes .Conclusion The way of injecting STZ adding high fat-feed can estabilish successfully the animal model of dia-betes with fatty liver.And this model was also in line with human charac-teristics of this desease .
