Effect of eEF1A1 inhibition by siRNA on apoptosis and the chemosensitivity of colon carcinoma cells HCT-116
10.13699/j.cnki.1001-6821.2015.17.023
- VernacularTitle:siRNA抑制真核翻译延长因子1 A1表达对结肠癌细胞凋亡和药物敏感性的影响
- Author:
Qi-Biao SU
1
;
Gui-Ning WEI
;
Hong-Jun LIU
;
Chen-Sheng LI
;
Li-Zhen LIAO
;
Jie ZHAO
;
Guo-Biao LI
;
Wei-Dong LI
Author Information
1. 广东药学院 健康学院
- Keywords:
siRNA;
eukaryotic translation elongation factor 1 A1;
colon carcinoma cells HCT -116;
apoptosis;
chemosensitivity
- From:
The Chinese Journal of Clinical Pharmacology
2015;(17):1777-1780
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of eukaryotic translation elongation factor 1A1 (eEF1A1) inhibition by siRNA on the apoptotic susceptibility , the chemosensitivity of colon carcinoma cells.Methods Four siRNA specifically targeting eEF1A1 were designed and synthesized in vitro and were transfected into colon carcinoma HCT -116 cells.Reverse transcription -polymerase chain reaction ( RT -PCR ) and Western blot assay were used to validate gene -silencing efficiency of eEF1A1.The cell apoptosis was assessed by flow cytometry ( FCM ).Cell growth state and 50% inhibition concentration ( IC50 ) of 5-fluorouracil ( 5 -FU ) and cisplatin was determined by MTT assay.Results siRNA specific to eEF1A1 can efficiently decrease the expression of eEF1 A1 in colon carcinoma HCT -116 cells.When eEF1A1 was inhibited , the reproductive activity of colon carcinoma HCT-116 cells was significantly lower than the control groups ( P<0.05).The study also showed that IC 50 of 5-FU and cisplatin on colon carcinoma HCT -116 cells treated by siRNA was decreased ( P<0.05).Conclusion The siRNA targeting eEF1A1 gene can effectively inhibit the expression of eEF1A1 gene, resulting in enhancing the apoptotic susceptibility , the chemosensitivity of 5-FU and cisplatin on colon carcinoma HCT -116 cells.
