Effect of tetramethylpyrazine on adjuvant arthritis by induction of apoptosis of synovial cells
10.13699/j.cnki.1001-6821.2015.17.020
- VernacularTitle:川芎嗪对佐剂性关节炎大鼠滑膜细胞凋亡的诱导作用
- Author:
Liu XUAN
1
;
Juan GU
;
Xue-Dong WANG
Author Information
1. 江南大学 附属无锡五院 中心实验室
- Keywords:
adjuvant arthritis;
synovial cells;
apoptosis;
tetrame-thylpyrazine
- From:
The Chinese Journal of Clinical Pharmacology
2015;(17):1766-1768
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tetramethylpyrazine ( TMP) on synovial cells of adjuvant arthritis ( AA ) , and the possible mechanism in the treatment of AA.Methods Adjuvant arthritis rat model was induced by a single intradermal injection of 0.1 mL of the complete Freund adjuvant into the right hind feet pads of the Spraugue Dawley(SD ) rats.AA rats were treated with various concentrations (30, 60,90 mg· kg -1 ) of TMP and collect the synovial cells.The prolifera-tion of synovial cells was measured by Methyl thiazolyl tetrazolium assay ( MTT).The protein expressions of Bcl -2 and Bax in the synovial cells were detected by Western blotting.The apoptosis rates of synovial cells were evaluated using flow cytometry ( FCM ) analysis.Results Compared with the model group , TMP could inhibit the proliferation of synovial cell in a dose-dependent manner, the expression of Bcl-2 on the groups with TMP treated was significantly increased and the expression of Bax was decreased.TMP can induce the apoptosis of synovial cells in vivo.Conclusion TMP can inhibit proliferation and induce apoptosis in synovial cells and the mechanism could be most likely to involve regulating and controlling the expression of Bcl -2 and Bax.
