Effects of α-asarone on activation of astrocyte of hippocampus in rat
10.13699/j.cnki.1001-6821.2015.17.018
- VernacularTitle:α－细辛醚对大鼠海马星型胶质细胞活化的影响
- Author:
Shang XIANG
1
;
Nong XIAO
;
Jing-Kun MIAO
;
Yang BI
;
Qi-Xiong CHEN
Author Information
1. 重庆医科大学 附属儿童医院
- Keywords:
α-asarone;
astrocyte;
glial fibrillary acidic protein;
lipopolysaccharide
- From:
The Chinese Journal of Clinical Pharmacology
2015;(17):1760-1762
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of α-asarone on acti-vation of astrocytes of rat hippocampus.Methods Rat status epilepticus ( SE) lasted for 1 hour.The activations of astrocyte in rat hippocampus of control group , model group and α-asarone treatment group were ob-served by Glial fibrillary acidic protein ( GFAP) immunohistochemistry.The astrocytes of rat hippocampus after passage were randomly assigned to control group , lipopolysaccharide ( LPS ) model group and α-asarone treatment group.Then the ability of cell proliferation were detected by CCK8.The GFAP were detected by Western -blotting .Results The most obvious activation of astrocyte in hippocampus of post -SE rats was observed at 1 d, then back to normal at 28 d after SE.Compared with model group , the activated levels of astrocyte in α-asarone treatment group were significantly decreased at 1,3,7 days after SE (P<0.05).Compared with model group , proliferation of astrocyte induced by LPS were significantly decreased in 50 μg· mL-1 and 100 μg· mL-1 ofα-asarone treatment groups and GFAP expression were significantly de-creased in 100 μg· mL-1α-asarone group (P<0.05).Conclusionα -Asarone could decreased the activation of astrocyte of rat hippocampus.
