Effect of triptolide on the proliferation, apoptosis and and H3K4me3 protein expression in multiple myeloma KM3 cell
10.13699/j.cnki.1001-6821.2015.17.017
- VernacularTitle:雷公藤内酯醇对多发性骨髓瘤KM3细胞增殖与凋亡和H3K4me3蛋白表达的影响
- Author:
Yan-Ping SHAO
1
;
Wen-Da LUO
;
Qun-Yi GUO
;
Zhen CAI
Author Information
1. 浙江大学 附属第一医院 血液科
- Keywords:
multiple myeloma;
triptolide;
KM3;
H3K4me3;
proliferation;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2015;(17):1757-1759,1785
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of triptolide on the proliferation, apoptosis and and H3K4me3 protein expression in multiple myeloma KM3 cell.Methods The MM KM3 cell line was cultured with triptolide.Cell proliferation was detected by MTT method .Apoptosis was evaluated by Annexin -V -FITC/PI -labeled flow cytometry and confocal microscopy.The expression of H3K4me3 in KM3cells was assayed by Western blotting and confocal microscopy.Results Triptolide had obvious inhibition on proliferation of KM 3 cells and showed a dose and time dependence.Triptolide had obvious inhibition on apoptosis of KM 3 cells,and with the increase of triptolide concentration , the apoptosis ratio increased gradually. The the total apoptosis rates were (48.97 ±1.78 )%, ( 53.72 ±2.21 )% and ( 60.75 ±2.43 )%.Confocal laser scanning microscopy showed H 3K4me3 accumulated in the nucleus, after 48 h intervention of 80 nmol· L-1 of triptolide, the mean value of fluorescence intensity of H3K4me3 in KM3 cells (21.96+0.34 ) was significantly lower than that in the control group ( 39.86 ±0.47 ) ( P <0.05 ) .Conclusion Triptolide inhibited cell proliferation, induced cell apoptosis, reduced the expression of H3K4me3 protein.
