Effect of apolipoprotein E genetic polymorphism on the efficacy of Tanshinol

VernacularTitle:载脂蛋白E基因多态性对丹参素调脂作用的影响
Author: Yu-Ping LUO ¹ ; Gao-Feng JIN ; Hong ZHANG ; Wen-Xiong SUN ; Yu-Qing XIONG
Author Information

1. 南昌大学临床药理研究所
Keywords: apolipoprotein E; gene polymorphism; Tanshinol; low den-sity lipoprotein receptor; 3 -hydroxy -3 -methylglutaryl coenzyme A reductase
From: The Chinese Journal of Clinical Pharmacology 2015;(17):1754-1756
CountryChina
Language:Chinese
Abstract: Objective To study the action of apolipoprotein E genetic polymorphism on the efficacy of Tanshinol.Methods Through detecting the expression of low density lipoprotein receptor mRNA and 3-hydroxy-3 -methylglutaryl coenzyme A reductase mRNA in L -02 cells by reverse transcription-polymerase chain reaction , to compare the effect of different genetic subtypes of ApoE on Tanshinol.Results Apolipopro-tein E can weaken the induction of low density lipoprotein receptor ex-pression caused by Tanshinol.The weakening extent of apolipoprotein E 2 (Arg112Cys)was stronger than apolipoprotein E3 and apolipoprotein E4 ( Cys 158 Arg) ( P<0.01 ).Apolipoprotein E also can enhance the inhi-bition of 3-hydroxy -3-methylglutaryl coenzyme A reductase expres-sion, especially apolipoprotein E4 (P<0.01).But apolipoprotein E2 or apolipoprotein E3 can not have statistical significance ( P >0.05 ).Conclusion Both 112 and 158 site might be the key site that have effect on the efficacy of Tanshinol.