Study on the effective mechanism of Plumula Nelumbinis extract against hepatic fibrosis in rats
10.13699/j.cnki.1001-6821.2015.17.015
- VernacularTitle:莲子心萃取物抑制大鼠肝纤维化的作用机制研究
- Author:
Zhi-Yong LIU
1
;
Jin YI
;
XIAO-Ming ZUO
Author Information
1. 赣南医学院 第一附属医院 血管乳腺科
- Keywords:
Plumula Nelumbinis extract;
hepatic fibrosis;
hepatic stellate cell;
tumor necrosis factor-α;
transforming growth factor-β1
- From:
The Chinese Journal of Clinical Pharmacology
2015;(17):1749-1753
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Plumula Nelumbinis extract ( PNE) on hepatic stellate cell activation and liver fibrosis in vitro and in vivo in rats model.Methods PNE impact on hepatic stellate cells was detected by cell experiments . The hydrogen peroxide content was determined in the cell by fluorescence analyzer.The ollagen deposition , nuclear factor -κB ( NF -κB ) activation , Iκbαphosphorylation ,α-SMA protein expression and gene transcription were detected by Western blot.Animals are divided into three groups , sham group , 0.7%carboxymethyl cellulose ( CMC ) was administrated; model group , Bile duct ligation in rats , 0.7%CMC was administrated;experimental group , Bile duct ligation in rats and PNE was administrated , twice a day from 3 th week.PNE impact on aspartate transaminase ( AST ) and alanine transaminase ( ALT ) was detected by colorimetric analyzer.The liver fibrosis scores,α-SMA and col1 α2 expression was detected by quanti-tative real -time polymerase chain reaction.Results Compared with blank group , PNE (80 μg· mL-1 ) can inhibit intracellular hydrogen peroxide content of hepatic stellate cells caused by TNF -α, alpha smooth muscle actin (α-SMA) protein expression, and intracellular NF-kappa B inhibitor-α(IκBα) phosphoryla-tion, and inhibit NF-κB activation and α-SMA protein expression.PNE (80 μg· mL-1 ) can significantly inhibit cell collagen production.In the experiment where Bile duct ligation ( BDL ) induced liver fibrosis in rats.Compared with model group,the use of PNE can reduce AST and ALT , decrease hepatic collagen content , and reduce α-SMA, mouse collagen type I Alpha 2 ( col1α2 ) mRNA expression.Conclusion PNE can inhibit liver fibrosis.
