Effect of microRNA -30 b on Sox4 protein and Pdcd4 expression in colorectal cancer cell lines
10.13699/j.cnki.1001-6821.2015.17.011
- VernacularTitle:微小 RNA－30 b对直肠癌细胞系松软蛋白4及程度细胞凋亡基因4表达的影响
- Author:
Fang XU
1
;
Hong YIN
;
Yan QIN
;
Fan WU
;
Ya-Nan WANG
;
Juan ZHANG
Author Information
1. 河北大学附属医院
- Keywords:
colorectal cancer cell line;
soft protein 4;
apoptosis gene 4;
targeted therapy;
microRNA-30 b
- From:
The Chinese Journal of Clinical Pharmacology
2015;(17):1736-1739
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA -30 b on the expression of Sox 4 and Pdcd4 in colorectal cancer cell lines.Methods Colon cancer HCT116 and SW480 cell lines cultured in 10%newborn bovine serum ,were divided into 2 groups trained in cell culture medium for 6 h.The experiment group were transfection by miRNA -30b, and cultured in cell culture medium for 6 h.The expression of Pdcd4 and Sox4 were determined by Western blot and polymerase chain reaction (PCR).Results Sox4 absorbance of the experiment group was lower , Pdcd4 was higher , compared with the model group ( P<0.01 ) .The ex-pression of Sox4 protein of the experiment was lower ,Pdcd4 was higher, compared with the model group ( P<0.01 ).Conclusion MicroRNA-30 b can significantly reduce the expression of Sox 4 protein in colorectal cancer cell lines ,Pdcd4 expression increased.
