Evaluation of P-glycoprotein and organic cation transporter on the transport of levofloxacin by Ussing chamber
10.13699/j.cnki.1001-6821.2015.17.010
- VernacularTitle:Ussing chamber技术评价 P－糖蛋白及有机阳离子转运体对左氧氟沙星跨胃黏膜转运的影响
- Author:
Yong-Mei HU
1
;
Shan-Shan ZHANG
;
Lei ZHANG
;
Xing-Ping HU
;
Jian-Ming XU
Author Information
1. 安徽医科大学 第一附属医院
- Keywords:
levofloxacin;
gastric mucosa;
Ussing chamber;
HPLC;
P-glycoprotein;
organic cation transporter
- From:
The Chinese Journal of Clinical Pharmacology
2015;(17):1732-1735
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of P-glycoprotein (P-gp) and organic cation transporter ( OCTs) on the transport of levofloxacin by using a model in vitro.Methods A model in vitro was established by rat gastric mucosa combined with Ussing chamber.The concentrations of levofloxacin collected from the transport assay were determined by HPLC and the transport parameters such as apparent permeability coefficient [Papp(M-S) and Papp(S-M)] and the ratio of Papp(S -M) versus Papp(M -S) were calculated and compared when the levofloxacin was used solely and co -used with P -gp inhibitor cyclosporin A or OCTs inhibitor cimetidine.Results When cyclosporin A was added , the Papp(S-M)/Papp(M-S) of levofloxacin decreased from 2.20 to 1.06 ( P<0.05).There was no significant difference compared with the blank group while cimetidine was added.Conclusion The reasults indicated that P-glycoprotein might be involved in the transport of levofloxacin in this model in vitro, while the organic cation transporter probably not participated in.
