Involvement of ubiquitin -proteasome pathway in down -regulation of P-glycoprotein in rat liver during acute inflammation
10.13699/j.cnki.1001-6821.2015.16.028
- VernacularTitle:泛素－蛋白酶体系统参与急性炎症状态下大鼠肝 P－糖蛋白表达下调
- Author:
Qi-Biao SU
1
;
Gui-Ning WEI
;
Lai-You WANG
;
Hua SU
;
Li-Zhen LIAO
;
Jie ZHAO
;
Guo-Biao LI
;
Wei-Dong LI
Author Information
1. 广东药学院
- Keywords:
ubiquitin -proteasome pathway;
P -glycoprotein;
down-regulation
- From:
The Chinese Journal of Clinical Pharmacology
2015;(16):1652-1655
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of P -glycoprotein ( P-gp) in rat liver during acute inflammation ( AI ) and the possible mechanism involved.Methods Twenty rats were randomly divided into two groups:model group (n=10) and control group (n=10).The rats in model group were treated by a single 5 mg? kg -1 intraperitoneal injec-tion of lipopolysaccharide ( LPS).Control rats received equivalent injec-tion of sterile normal saline.The levels of P-gp, ubiquitinated P-gp and 26 S proteasome activity in both groups were analyzed by Western blot, immunoprecipitation and fluorescence detection, respectively. Results Compared to the control groups, ubiquitination levels of liver P-gp and 26S proteasome activity in model groups were significantly in-creased ( P<0.01 ) , accompanied by an decrease of liver P-gp protein expression level. Conclusion The participation of the ubiquitin -proteasome system in down-regulation of liver P-gp expression levels under AI conditions.
