Lead tolerance of metallothionein -overexpressed human adipose -derived mesenchymal stem cells
10.13699/j.cnki.1001-6821.2015.16.025
- VernacularTitle:金属硫蛋白修饰人脂肪来源间充质干细胞对铅的耐受性研究
- Author:
Yun ZHANG
1
;
Xi-Luan JI
;
Zhao-Xia LUO
;
Shun YANG
;
Xiao-Lei LIU
;
Jie-Ming LI
;
Liang XIE
;
Shu JIANG
Author Information
1. 深圳市茵冠生物科技有限公司
- Keywords:
metallothionein;
lentiviral vector;
human adipose-derived mesenchymal stem cells;
lead poisoning
- From:
The Chinese Journal of Clinical Pharmacology
2015;(16):1642-1644
- CountryChina
- Language:Chinese
-
Abstract:
Objective The lentiviral vector was recombined with metal-lothionein ( MT) gene to identify the MT overexpression in human adi-pose-derived mesenchymal stem cells ( hADSCs) after transfection and then to study the lead tolerance of genetically modified hADSCs with MT (MT-hADSCs).Methods The recombinant plenti-CMV-MT2A-EYFP vector was constructed with pLenti -CMV -hChR 2 ( E123 T -H134R)-EYFP and MT2A gene for transfecting hADSCs to obtain the MT-hADSCs.The overexpression of MT in hADSCs was identified by immunofluorescence assay.The MTT method was used to assess the cell viability of hADSCs, hADSCs transfected with empty vector, and MT-hADSCs, all of which were treated with lead acetate.Results The re-combinant plenti -CMV -MT2A -EYFP was successfully constructed and transfected into hADSCs.The overexpression of MT was positively detected in the MT -hADSCs.The tolerance of MT-hADSCs to lead was significantly higher than the hADSCs and hADSCs transfected with empty vector.Conclusion MT can significantly increase the tolerance of hADSCs to lead, indicating that MT can reduce the cytotoxicity of lead.The experimental results from this study provide more evidences for further study of using MT-hADSCs to treat lead poisoning.
