Effect of sperm-associated antigen 9 on migration and invasion abilities of human osteosarcoma cells

VernacularTitle:精原相关蛋白9干扰对人骨肉瘤细胞迁移侵袭影响的实验研究
Author: Wen-Lai ZHOU ¹ ; Xiao-Rong YANG ; Hao WU
Author Information

1. 湖北省恩施州中心医院骨科
Keywords: sperm-associated antigen 9; siRNA; migration; invasion; osteosarcoma
From: The Chinese Journal of Clinical Pharmacology 2015;(16):1638-1641
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of sperm -associated antigen 9 ( SPAG9 ) on migration and invasion abilities of human osteo-sarcoma U2OS cells.Methods siRNA was used to decrease SPAG9 expression, and then cells were subjected to CCK -8 assay, wound healing assay and transwell assay to detect siSPAG9 on cell migration and invasion abilities. Western blot and Gelatin zymography were used to detect matrix metalloproteinase-2 ( MMP-2) and matrix metalloprotei-nase-9 ( MMP -9 ) expression and activities. Results siRNA for SPAG9 significantly decreased SPAG9 mRNA and protein expression. siSPAG9 had no effect on the proliferation of U2OS cells, but significant-ly inhibited its wound healing, migration and invasion abilities. The activities and expression of MMP-2 amd MMP-9 were also suppressed by siRNA for SPAG9.Conclusion Depletion of SPAG9 results in the inhi-bition of migration and invasion abilities of human osteosarcoma U2OS cells.