Effects of N-acetylcystein on cardiac hypertrophy in pressure-overload rats and the relationship with inflammation
10.13699/j.cnki.1001-6821.2015.16.019
- VernacularTitle:氮乙酰半胱氨酸对压力负荷高血压大鼠心肌肥厚的影响及其抗炎机制
- Author:
Fu-Jun SHANG
1
;
Jie-Pin WANG
;
Hui LIU
;
Xue LI
;
Lian-You ZHAO
Author Information
1. 第四军医大学 唐都医院
- Keywords:
cardiac hypertrophy;
inflammatory cytokines;
antioxidant;
pressure-overload
- From:
The Chinese Journal of Clinical Pharmacology
2015;(16):1622-1625
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of N -acetylcystein ( NAC) on the cardiac hypertrophy in pressure -overload rats and the relationship with the expression of inflammatory cytokines.Methods The SD rats were randomly divided into four groups:sham group, model group , drug group in sham and drug group in model.Pressure-overload rat model was established by abdominal aortic coarctation.Rats in drug group were treated with NAC ( 0.2 g? kg-1? d -1 , ig ) for 8 weeks. Cardiac hypertrophy was evaluated by heart histological analysis and echocardiography.The collagen content was determined by chromatome-try.RT-PCR and ELISA were used to determine the expression of in-flammatory cytokines.The production of reactive oxygen species ( ROS) were measured by Fenton reaction.Results Compared with sham rats, blood pressure, left ventricular weight index( LVWI) , myocyte diameter, left ventricular wall thick(LVWT) as well as the collagen content( CC) in model rats significantly elevated ( P <0.05 ) .In model rats treated with NAC, LVWI, LVWT and CC were significantly decreased as well as the production of ROS ( P <0.05 ) . Furthermore, the expression of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)but IL-10 was elevated in myocardium of pressure-o-verload rats, which was inhibited by NAC( P<0.05).NAC had no effects on the expression of IL-10.Conclusion NAC could improve the cardiac hypertrophy induced by pressure-overload.The effects of NAC probably relates to the decrease of pro-inflammatory cytokines expression in rat myocardium.
