Experimental study of Zuogui Wan on improvement memory in cerebral ischemia model rats of kidney deficiency induced by panic
10.13699/j.cnki.1001-6821.2015.16.018
- VernacularTitle:左归丸改善惊恐伤肾脑缺血大鼠记忆力的实验研究
- Author:
Hong LIU
1
;
Yan JIA
;
Shi-Xiao SUN
;
Di HAO
;
Shuang LIU
;
Yang YE
;
Li-Li ZHONG
Author Information
1. 黑龙江中医药大学
- Keywords:
Zuogui Wan;
memory;
brain -derived neurotrophic factor;
kidney deficiency induced by panic;
cerebral ischemia
- From:
The Chinese Journal of Clinical Pharmacology
2015;(16):1618-1621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Zuogui Wan on the memory of the rats with panic injury and the neuroprotective effect . Methods Forty female Waster rats were randomly divided into 4 groups: sham group, model -brain group, model -kidney group, test group.Middle cerebral artery occlusion( MCAO) was operated in rats ex-cept sham group,and ischemia-reperfusion were executed 2 hours after MCAO.The model-kidney group was threatened by the cat in 10 days before MCAO operation.The rats in the test group orally 1.62 g? kg -1 Zuogui Wan Decoction 2 mL at the time of 3 days before MCAO opera-tion , 1 times a day continuously for 10 days.The other 3 groups were giv-en equal volume of saline.The memory in each group rats was detected by novel object recognition task.The neuron injury in rats was observed by HE staining.The expression of brain -derived neurotrophic factor ( BDNF ) was assessed by immunohistochemistry and Western blot.Results Compared with sham group, memory decline after MCAO in model -brain group rats.Compared with the model -brain group, memory impairment was aggravated induced by ischemia and downregu-lated of expression of BDNF in model-kidney group,which was statisti-cally difference ( P<0.05 ) .Compared with the model-kidney group memory in rats were significantly improved and the expression of BDNF was increased in the test group,which was statistically difference (P<0.05).Conclusion Kidney deficiency induced by panic can significant aggravate memory impairment after cerebral ischemic injury.Zuogui Wan can improve memory ability of rats of kidney deficiency induced by panic after ischemia,which is likely achieved by upregulation of the ex-pression of BDNF.
