Effect of the momordica charantia total saponin on the key factors of ameliorating insulin resistance in type 2 diabetes mellitus rats
10.13699/j.cnki.1001-6821.2015.15.017
- VernacularTitle:苦瓜总皂苷对改善2型糖尿病大鼠胰岛素抵抗关键因子的影响
- Author:
Chun-Yu MA
1
;
Hong-Yu YU
;
Hui-Jiao WANG
;
Li-Jing GENG
Author Information
1. 辽宁医学院
- Keywords:
momordica charantia total saponin;
type 2 diabetes;
insulin resistance;
protein kinase B;
adenosine monophosphate activated protein kinase
- From:
The Chinese Journal of Clinical Pharmacology
2015;(15):1522-1525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect mechanism of momordica charantia total saponin ( MCTS)′s ameliorating insulin resistance through discussing MCTS′s influence of protein kinase B ( Akt -2 ) and adenosine monophosphate activated protein kinase ( AMPK ) in type 2 diabetes ( T2DM ) rats.Methods T2DM models were built with the method of higher lipid and sugar diets and injection of a low dose of streptozotocin.Rats are divided into normal group, model group, control group and three dosage test groups.The positive control group and three dosage test groups rats were administrated with 2 mL metformin (50 mg· kg-1) and MCTS (100, 200, 400 mg· kg -1) per day for 8 weeks.Fasting serum glucose and insulin were detected with biochemical analyzer and ELISA method.The Akt-2 and AMPK′s mRNA and the activated protein were measured by RT-PCR and Western blot method.Results The homeostasis model assessment of insulin resistance ( HOMA-IR) reduced and the mRNA and protein expression amount of Akt-2 and AMPK increased obviously in all test groups compared to the model group.Conclusion The ameliorating insulin resistance mecha-nism of MCTS maybe caused by up-regulating the mRNA and protein of Akt-2 and AMPK.
