Effect of Langchuangqing granule on the secretion of IL-6,IFN-γin rats spleen lymphocytes and the expression of apoptosis gene p53,C-myc
10.13699/j.cnki.1001-6821.2015.14.023
- VernacularTitle:狼疮清颗粒对脾淋巴细胞分泌白细胞介素6与干扰素γ及凋亡基因 p53、C－myc的影响
- Author:
Tao LIANG
1
;
Lü JI
;
Shan GENG
;
Ling-Ling ZHOU
;
Xiao-Li ZHAO
;
Bing YU
Author Information
1. 南京中医药大学药理教研室
- Keywords:
Langchuangqing granule;
lymphocytes;
cytokine;
apoptosis gene
- From:
The Chinese Journal of Clinical Pharmacology
2015;(14):1432-1434
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Langchuangqing granule on the secretion of IL-6 ,IFN-γin rats spleen lymphocytes and apopto-sis gene p53 , C -myc. Methods Rats were administered with Langchuangqing granule 22.8 g? kg -1 by gavage for 3 days.At the same time, spleen cells were separated in ICR mouse.The large dose group with 90%volume fraction, middle dose group with 50%volume fraction, small dose group with 20% volume fraction, positive drug group with spleen cell suspension plus dexamethasone, control group with of spleen cell suspension plus blank serum were included this study. Spleen lymphocytes in rats were cultured for 24 h in vitro.Levels of IL-6 and IFN-γwere measured by radioimmunoassay in rats spleen lymphocytes supernatant.The expression of apoptosis gene p53 and C -myc were detected by immunohistochemical method.Results Levels of IL-6 and IFN-γwere significantly decreased in serum containing Langchuangqing granule in large, middle dose group and dexamethasone compared with control group. The expression of apoptosis gene p53 was significantly decreased in all groups.The expression of apoptosis gene C-myc were sig-nificantly decreased in the large dose group (P<0.05).Conclusion The mechanism of Langchuangqing granule can inhibite levels of IL-6, IFN-γand the expression of apoptosis gene p53 and C-myc.
