Effects of cAMP receptor protein regulator on bacterial virulence and biofilm formation
10.13699/j.cnki.1001-6821.2015.14.018
- VernacularTitle:环磷酸腺苷受体蛋白调控子对细菌毒力及生物膜形成的影响
- Author:
Bei-Bei CHEN
1
;
Gang YANG
;
Xun YANG
;
Qin OU
Author Information
1. 武汉市中心医院呼吸内科
- Keywords:
Klebsiella pneumoniae;
cAMP receptor protein regulator;
bacterial virulence;
biofilm formation
- From:
The Chinese Journal of Clinical Pharmacology
2015;(14):1415-1417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effects of cAMP receptor protein(CRP) regulator on the virulence and biofilm formation for the Klebsiella pneumoniae.Methods From August 2012 to February 2014, selected120 Klebsiella pneumoniae strains for PCR detecting expression of CRPregulators, while selected piperacillin, piperacillin +tazobactam, amikacin,ceftazidime, ceftriaxone, cefoperazone +sulbactam, levofloxacinand other antibacterial drugs for the resistance testing and analysis.Inthe biofilm formation experiment, were given the detailed analyzed of theregulation of relative positive expression strains in ordinary liquid cultureand biofilm state situation.Results PCR detection positive rate of CRPregulators were 33.3%, while the CRP regulatory sequences flankingPCR positive strains also amplified target sequence.The resistance ofCRP regulator positive strains for ceftriaxone, levofloxacin, amikacin andceftazidime were higher than the CRP regulators negative strains thatdifference were statistically significant (P <0.05).The CRP regulatorexpression in the biofilm culture group was 0.28 ±0.04, so that was0.08 ±0.05 in the ordinary liquid culture group, the difference betweentwo groups was statistically significant (P <0.05).Conclusion Theexpression of CRP regulators for the Klebsiella pneumoniae is favor ofreducing the bacterial virulence and promote biofilm formation, thereby i nc rease resistance to drugs-related antimicrobial.
