Effects of combined tacrolimus ointment and narrow band ultraviolet B on the changes of the level of interleukin-10 and tyrosinase in Guinea pig with vitiligo
10.13699/j.cnki.1001-6821.2015.13.021
- VernacularTitle:他克莫司外用联合窄谱中波紫外线对白癜风豚鼠的白细胞介素-10及酪氨酸酶水平变化的影响
- Author:
Ya-Ning JIAO
1
;
Li ZHANG
;
Jing-Xia WANG
;
Jun-Hui LI
;
Xiao-Shuai LIU
;
Zhen-Dong ZHONG
;
Li XIA
;
Li LIANG
Author Information
1. 宁夏医科大学 总医院 皮肤科
- Keywords:
vitiligo;
tacrolimus ointment;
narrow band ultraviolet B;
interleukin-10;
tyrosinase
- From:
The Chinese Journal of Clinical Pharmacology
2015;(13):1289-1291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of combined narrow band ultraviolet B ( NB-UVB) and tacrolimus on the changes in the level of tyrosinase and interleukin -10 ( IL -10 ) in Guinea pig with vitiligo. Methods Animal models with vitiligo were built first .And then all the animal models were randomly divided into six groups of eight according to the principle of weight balance: normal group , model group , control group(2 times per a day), medication alone group (0.03% tacrolimus ointment skin smear , 2 times per a day ) , light alone group ( white spot area use NB -UVB irradiation , once every other a day ) , combination group(topical tacrolimus combination NB -UVB irradiation, frequency , method and time same to medication alone group and light alone group ). Colorimetric method and ELISA method were used to detect the changes in the level of tyrosinase in serum and IL -10 in skin tissue homogenates and serum respectively , before and after the administration and irradi-ate.Results No significant changes in the level of tyrosinase in serum were observed before and after combined administration of NB -UVB and tacrolimus in animal models with vitiligo .While IL-10 contents in skin tissue homogenates and serum were significantly higher than that before administration , and the difference was statistically significant (P<0.01).Conclusion Combined treatment of NB-UVB and tacrolimus can significantly improve the level of IL -10 in skin tissue homogenates and serum for animal models with vitiligo .
