Research on degradation of dural sealant material in vivo
10.13699/j.cnki.1001-6821.2015.13.014
- VernacularTitle:脑硬膜防渗医用涂敷系统体内降解实验研究
- Author:
Pei YU
1
;
Xi YANG
;
Jing-Jing ZHANG
;
Jian-Jie JIAO
;
Qin LI
;
Jing LI
;
YU-Qing YIN
;
Meng-Yao AN
;
Jian-Shi LAO
Author Information
1. 天津医科大学 基础医学院 药理学教研室
- Keywords:
PEG15000;
high performance liquid chromatography;
dural sealant material;
degradation in vivo
- From:
The Chinese Journal of Clinical Pharmacology
2015;(13):1265-1267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of polyethylene glycol 15000 ( PEG15000 ) , the main degradation product of medical coating system inside the body by using high performance liquid chromatography -evaporative light scattering detector ( HPLC-ELSD ) and to evaluate its degradation rule.Methods The medical coating system was implanted into the back of rats , and these rats were executed by cervical vertebra dislocation zero, one, two, three, four, five, six, seven, eight, nine, ten, twelve weeks (n=6) after operation, respectively and washed with phosphate buffer to collect the rest gel and washing fluid , then HPLC-ELSD was used to determine the content of PEG 15000 in the rest gel and washing fluid .Results Gel in rats was intact until the end of the third week , presented as colorless transparent pieces until the sixth week, and then transformed to liquid state , presented as water until the ninth week and not seen by naked -eye until the twelfth week.The content of PEG15000 in the rest gel was decreased progressively with the extension of experiment weeks .Conclusion The degradation rate of gel inside the body of rates presents the rule of one fast and one slow;which conforms to the requirements in physiological process of dural defects healing.
