LC-MS/MS method for the detection of platelet-activating factor C-18 and its acetylhydrolase
10.13699/j.cnki.1001-6821.2015.12.033
- VernacularTitle:血小板活化因子 C－18及其乙酰水解酶的液质联用检测方法
- Author:
Wei LIU
1
;
Xin XIONG
;
Fu-Yu SONG
;
Li YANG
;
Suo-Di ZHAI
Author Information
1. 北京大学第三医院药剂科
- Keywords:
platelet-activating factor;
LC-MS/MS;
platelet-activating factor-acetylhydrolase
- From:
The Chinese Journal of Clinical Pharmacology
2015;(12):1191-1193,1201
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a liquid chromatography -mass spectrometry ( LC-MS/MS) method for the analysis of platelet-activa-ting factor C-18(PAF C-18)and its acetylhydrolase in human plasma. Methods A convenient extraction procedure using trichloromethane, methanol and water as the extract solvent was used.The separation was carried out on Agilent XDB C -18 analytical column.Mobile phase A consisted of methanol -ammonium acetate(1 mmol? L-1 )-acetonitrile ( 57 ∶23 ∶20 ) , and mobile phase B was methanol containing 1 mmol? L-1 ammonium acetate.Gradient elution was used.The speci-ficity, linearity and limit of quantification, precision and recovery, ma-trix effect and stability were assessed.Results The assay was reproduci-ble within the linearity range from 0.5-25.0 ng? mL-1 ( r=0.998 4 ) . The relative standard deviation( RSD) of intra-day precision were below 10%, with recovery rate ranged from 67.0% to 82.1% and the matrix effect ranged from 115.3%to 116.9%.PAF C-18 was fully degraded over 90 min at room temperature.Conclusion This method provides a sensitive, special and convenient approach for the clinical analysis of PAF C-18, as well as the indirect measurement of PAF-acetylhydrolase activity by analyzing the concentration decrease of an exogenous PAF.
